Glucose Determination in Blood: A Centrifugation and Spectrophotometry Technique

🔬 In the video, we observe the process of glucose determination in blood samples in a clinical laboratory.

⏳ After obtaining the sample, it is left to rest in a rack for 30 minutes, followed by centrifugation at 3000 for 5 minutes.

💉 This method is commonly used to measure glucose levels in blood and is an important test in clinical diagnostics.

🔬 The process of determining blood glucose levels involves centrifuging a blood sample for 5 minutes at 3000 revolutions.

🩸 After centrifugation, the blood components separate, and the serum is collected for analysis.

🧪 Prior to the procedure, it is essential to prepare the necessary equipment and reagents and properly label the test tube.

🩸 The video is about determining blood glucose levels.

🧪 The process involves using a pipette to collect 10 microliters of blood, followed by adding 1000 microliters of glucose reagent.

📋 The steps include absorbing the blood sample with the pipette, disposing of the pipette tip, and then adding the glucose reagent.

The video is about measuring glucose levels in blood.

The process involves taking a blood sample and placing it in a test tube.

The glucose test is done using a spectrophotometer with specific wavelength and light passage requirements.

🔍 Before reading the sample, calibrate the equipment and determine the glucose level.

💡 The factor for glucose is 235, and the sample is placed in the spectrophotometer for analysis.

🧪 The glucose level is measured in milligrams per deciliter.

🩸 The video is about determining blood glucose levels using a blood glucose meter.

📊 The normal range for blood glucose levels is between 70 to 110 mg/dL.

✅ The test result in the video was within the normal range, with a reading of 76 mg/dL.